Esther's debate with the Sages on the canonization of the Megillah: Megillah 7a
Whether the Megillah was first composed/written with divine inspiration: Megillah 7a [2x]
Whether the scroll has the level of holiness to include it in the edict which created a level of impurity for hands which touch it: Megillah 7a
Divine acceptance of the scroll, after the Jews accepted it: Megillah 7a