Whether an engaged woman can become a Sotah through seclusion with another man: Kiddushin 27b
Whether a convert can become a Sotah: Berachot 19a [Rashi there is tamuah]
Whether a freed eved can become a Sotah: Berachot 19a
Procedural Issues
How many scholars accompany husband and wife to the court, to ensure that the husband will not have sexual relations with his wife en route: Sotah 7a, 25a
Why we require scholars, as opposed to standard witnesses, to accompany the husband and wife to court: Sotah 7a
How many witnesses to an actual sexual act between a wife and another man are needed, in order that she not be allowed to go through the Sotah rite: Sotah 2a-b, 3a, 31a
What happens if there are witnesses to an actual sexual act, but they are not available: Sotah 6a-7a
Other Issues
The punishment of a Sotah matches her acts: Sotah 8b [2x], 8b-9a, 9a-9b, 9b
At what point in the Sotah process a woman who is married to a Kohen is no longer eligible for levirate marriage, if her husband dies without bearing children: Sotah 2a, 5b-6a
The Torah's view of a man who marries a woman who was a Sotah while married to her first husband: Sotah 5b-6a