Assuming the Tithe was separated from a Trusted Person's produce: Pesachim 4b, 9a
Assuming that an unlearned person would take the tithe himself from a cheap item: Pesachim 42b
Tithe Separation
Requiring that one's intent and Expression be identical: Pesachim 63a
Separating on Produce from one year, for produce of a different year: Rosh HaShanah 12a-b
What happens if a person separates too much: Eruvin 50a
What happens if a person says "Half of each of these kernels is the tithe": Eruvin 50a
Approximating the percentage: Beitzah 13b
Who Eats It
Levites, vs. Levites and Kohanim, vs. Regular Jews: Ketuvot 26a
Ezra's fine against the Levites, removing their exclusive rights to the tithe: Ketuvot 26a
The Heavenly Court agreed with Ezra's enactment: Makkot 23b
Eating it while it is Impure: Pesachim 24a
Eating it as Matzah on Pesach: Pesachim 35a