When the kohen who would collect the ashes raised or submerged the sink for washing, that signaled the other kohanim that the time for washing had arrived: Zevachim 21b
The ashes must be left by the Altar: Pesachim 26a; Temurah 34a; Keritot 6a
How the ashes must be placed down: Pesachim 26a, 27b; Temurah 34a
For one who wants to perform Temple Service in the morning, need he wash again if he already washed for terumat hadeshen after the rooster crowed that morning: Zevachim 20a-b