Viewing this washing as an official service in the Beit haMikdash: Zevachim 19b
For one who wants to perform Temple Service in the morning, if he has been working in the Temple all night: Pesachim 2b; Zevachim 19b-20a
For one who wants to perform Temple Service in the morning, if he already washed for terumat hadeshen after the rooster crowed that morning: Zevachim 20a-b
For one who wants to perform Temple Service at night, if he was already working at the end of the day: Zevachim 19b-20a
The fundamental reason to require re-washing is hesech hadaat, distraction from guarding one's purity: Zevachim 20b
Need one wash for entering the Beit haMikdash if he does not intend to perform a service ["biah reikanit"]: Zevachim 19b
Need one wash for entering the Beit haMikdash to perform a non-mandatory service: Zevachim 19b-20a
Need one wash again if he steps out of the Beit haMikdash: Zevachim 20b
Need one wash again if he sticks his hands out of the Beit haMikdash: Zevachim 20b
Need one wash again after urination or defecation: Zevachim 20b
The water
Requiring a reviit of water: Zevachim 21b-22a
Whether liquid clay counts toward the minimum amount of water: Zevachim 22a
Whether red worms count toward the minimum amount of water: Zevachim 22a
The washing
What time in the morning the kohanim washed: Zevachim 20a-b, 21b
When the kohen who would collect the ashes raised or submerged the sink for washing, that signaled the other kohanim that the time for washing had arrived: Zevachim 21b
The need to wash with a kli sharet [Beit haMikdash implement], inside the Beit haMikdsah: Zevachim 20b, 22a
Washing using the base [kan] of the sink: Zevachim 22a
Whether the right limbs are washed together and the left together, or all of the limbs are washed together: Zevachim 19b
Whether the kohen may sit, or be supported, during the washing and during general service: Zevachim 19b
How one re-washes should his hands become impure: Zevachim 20b [2x]
How one re-washes should his whole body become impure with a one-day impurity right before sunset: Zevachim 20b-21a
Does immersion of one's hands and feet in the Beit haMikdash sink count as washing: Zevachim 21a
Does immersion in a mikvah count as washing: Zevachim 20b, 21a
Shelo rechutz yadayim v'raglayim - Performing service in the Beit haMikdash without proper washing
Disqualification from performing the services of korbanot: Zevachim 17b-18a, 19b
Penalty for performing a service: Zevachim 23b-24a
Performing kabbalat hadam: Zevachim 15b